肥大细胞稳定剂对重症急性胰腺炎大鼠胰腺保护作用的实验研究

Experimental Study of Protective Effects of Mast Cell Stabilizer on Pancreas of Rats with Severe Acute Pancreatitis

目的研究肥大细胞(MC)稳定剂酮替芬对重症急性胰腺炎(SAP)大鼠胰腺的保护作用。方法SAP大鼠制模前或制模后30min给予酮替芬,制模后3h及6h取胰腺组织行HE染色计算病理评分,甲苯胺蓝及免疫组化染色观察MC数,酶显色法测定髓过氧化物酶(MPO)活性,了解中性粒细胞(PMN)聚集程度,Western Blot法检测MC类胰蛋白酶(MCT)表达,并与未用酮替芬处理的SAP大鼠进行对比。结果酮替芬处理组胰腺组织较未处理组病理损害减轻,MC数、MPO活性及MCT表达均明显下降。结论MC早期即被激活并参与SAP的致病作用。预防性或SAP早期应用酮替芬,可明显减轻胰腺的损害。

Objective To investigate the protective effects of mast cells on pancreas of rats with severe acute pancreatitis （SAP）. Methods Ketotifen was injected intraperitonealey 30 min before or after SAP model, 3 h and 6 h after operation, pancreas was removed to make pathologic score, to count the number of MC by the method of toluidine blue staining and immunohistochemistry, to measure the MPO activation, the activation level of tryptase by western blot, and compared with those of SAP rats without ketotifen treatment. Results Pretreatment or early using of ketotifen after SAP can decrease the damage of pancreas, decrease the MC number and the level of MPO and tryptase. Conclusion MCs are activated in the early stage of SAP, and induce damage to pancreas. Pretreatment with or early using of ketotifen can decrease the severity of pancreas damage in SAP.