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重症急性胰腺炎大鼠空肠酶组织化学的变化

Changes of Enzymes Activities of Jejunum Mucosa in Severe Acute Pancreatitis in Rats
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摘要 目的观察重症急性胰腺炎(SAP)时空肠组织酶活性的变化。方法将大鼠随机分为假手术组(SO组,开腹后牵拉十二指肠、胰腺)、SAP组(用逆行胰胆管注射牛磺胆酸钠复制SAP模型)、谷氨酰胺(Gln)治疗组(SAP+Gln组,制作SAP模型后用Gln治疗),于术后24h、48h、72h分批处死动物,用组织化学染色法对空肠组织中碱性磷酸酶(ALP)、ATPase酶、细胞色素C氧化酶(CCO)、琥珀酸脱氢酶(SDH)进行染色,观察比较酶活性变化及其规律。结果SAP组ALP、ATPase、CCO、SDH活性明显降低,且随病程发展呈进行性下降,经过Gln治疗后酶的活性显著恢复。结论SAP时肠道功能随病情进展而下降,谷氨酰胺具有保护肠道功能的作用。 Obieetive To investigate the changes of enzymes activities of jejunum in rats with severe acute pancreatitis (SAP). Methods Rats were divided randomly into 3 groups:sham operation group (SO), SAP group, and treatment group with Gin (SAP+ Gin) . SAP model was reproduced by retrograde infusion of sodium taurocholate solution into the hiliopancreatic duct. In addition, the SAP+GIn group was feeded with glutamine after operation. Tissues of jejunum were taken at 24 h, 48 h and 72 h respectively after operation, and the activities of alkaline phosphatase, ATPase, cytochrome oxidase, succinate dehydrogenase were studied using enzyme-histochemistry staining. Results The activities of ALP,ATPase,CCO,SDH decreased significantly in SAP group,while Gin could increase them significantly. Conclusion It suggest that the intestinal function was impaired in SAP. Gin could protect intestinal function.
出处 《首都医科大学学报》 CAS 2006年第3期347-350,共4页 Journal of Capital Medical University
关键词 急性重症胰腺炎 酶组织化学 空肠 大鼠 sever acute pancreatitis enzyme-histochemistry jejunum rat
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