摘要
目的：观察平阳霉素诱发的大鼠白内障模型晶体上皮细胞ＤＮＡ损伤及修复的动态过程。方法：单细胞电泳法（Ｓｉｎｇｌｅｃｅｌｌｇｅｌａｓｓａｙ，ＳＣＧ）。结果：首次注射平阳霉素１２ｈ即出现严重的ＤＮＡ单链断裂（ＤＮＡｓｉｎｇｌｅｓｔｒａｎｄｂｒｅａｋｓ，ＳＳＢ），直至白内障产生，ＳＳＢ仍很严重，该ＤＮＡ损伤较其它模型更严重，其晶体囊内容物在致障过程中出现明显的液化与崩解。结论：平阳霉素性白内障模型在致障过程中有较其它模型更严重的ＤＮＡ单链损伤，且可能有不尽相同的机理存在。
Objectlve:To study DNA damage and repair kinetics in the rat’s Pingyangmycin-induced cataract model.Metbods:Single Cell Gel Assay(SCG)technique.Results:It was observed that the very obvious DNA migration happened just 12 hours after the first injection of Pingyangmycin and continued during cataractogenesis.Conclusion:The result suggested that the mechanism of the cataract formation induced by Pingyangmycin was different from those in cataract induced by other inducers.
出处
《北京医科大学学报》
CSCD
1996年第3期177-179,共3页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金
关键词
修复
平阳霉素
白内障
DNA
Headings DNA repair
cataract/chem ind
Pingyangmycin/adr eff
DNA damage
