骨形态发生蛋白/碱性成纤维细胞生长因子复合材料修复关节软骨缺损的实验研究

Experimental Study on Repair of Cartilage Defects with Combined BMP/bFGF Material

目的探讨骨形态发生蛋白(bone morphogenetic protein,BMP)碱性成纤维细胞生长因子(basic fibroblast growthfactor,bFGF)复合材料在关节软骨缺损修复过程中的作用,为内源性修复关节软骨缺损提供实验基础。方法选用兔作为实验动物造成双侧股骨髁间窝全层软骨缺损,分别采用不同的材料(rhBMP2/PVP组、bFGF/PVP组、rhBMP2/bFGF/PVP组、PVP组)进行缺损修复,光镜及电镜下观察修复效果。结果术后8周rhBMP2/bFGF/PVP组软骨缺损已基本修复,12周时软骨修复更加光滑、完整。其余组软骨缺损修复效果不如rhBMP2/bFGF/PVP组。结论BMP具有诱导形成软骨的活性,与bFGF的复合物能增强软骨细胞增生,较单独应用BMP效果好,是生物学修复关节软骨缺损的良好材料。

Objective To study on repair of articular cartilage defects with combined bone morphogenetic protein（BMP）/ basic fibroblast growth factor （bFGF） material in order to supply experimental basis for repairing cartilage defects biologically. Methods The rabbit were chosen and full thick defects of cartilage were made in intercondylar fovea of the femur, the defects were repaired with different materials （rhBMP2/PVP, bFGF/PVP, rhBMP2/bFGF/PVP, PVP）, and the results were observed with histology and electron microscope. Results 8 weeks after operation, the defects of cartilage had almost repaired in the rhBMP2/bFGF/PVP group, 12 weeks after operation, the cartilage were smoother, the results of other groups were not so good as this group. Conclusion BMP had the ability to induce cartilage formation,combined BMP/bFGF material increased cartilage cell proliferation,and had the better result than using BMP alone, combined BMP/bFGF material was good for repair of articular cartilage defects.