柠檬酸盐和EDTA缓冲液对弥漫性大B细胞淋巴瘤Ki-67和bcl-2抗原修复的比较

Comparison of citrate with EDTA buffer in antigen retrieval of Ki-67 and bcl-2

目的探讨柠檬酸盐和EDTA缓冲液对弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphomas,DLBCL)中Ki-67和bcl-2抗原修复的比较。方法运用高压锅热抗原修复法,选择柠檬酸盐和EDTA两种缓冲液同时进行抗原修复,比较了78例弥漫性大B细胞淋巴瘤Ki-67和43例DLBCL bcl-2的免疫组化染色应用效果。结果免疫组化染色采用EDTA与柠檬酸盐缓冲液相比,Ki-67指数78例中有76例明显提高,两者差异有统计学意义(P<0·01);bcl-2染色43例中有13例表达明显提高,两者差异有统计学意义(P<0·01)。结论在实际免疫组化染色中,EDTA缓冲液较柠檬酸盐缓冲液能极大地提高弥漫性大B细胞淋巴瘤的Ki-67和bcl-2抗原的表达。

Purpose To compare citrate with EDTA buffers in antigen retrieval for Ki-67 and bcl-2 staining in diffuse large B-cell lymphomas （DLBCLs）. Methods Using pressure cooker heat-induced antigen retrieval, citrated and EDTA buffers were compared in Ki67 immunostaining in 78 cases and bcl-2 in 43 cases of DLBCL synchronously, Results The Ki-67 index was enhanced in 76 of 78 cases, when EDTA buffer was compared with citrate buffer, The difference was statistically significant （P 〈 0. 01 ）, The expression of bcl-2 protein was enhanced in 13 of 43 cases, and the difference was also statistically significant （P 〈 0. 01 ）, Conclusions In clinical practice, the use of EDTA buffer could enhance the immunohistochemical staining of Ki-67 and bcl-2 protein in DLBCLs significantly, compared with citrate buffer.