尖锐湿疣患者皮损、非皮损表皮中TGF-α和EGFR的表达

Expression of TGF-α and EGFR in condyloma acuminatum

目的:探讨转化生长因子(TGF-α)、表皮生长因子受体(EGFR)在尖锐湿疣(CA)发病机制中的作用。方法:采用免疫组化二步法检测15例CA损害中TGF-α、EGFR的表达情况,并与CA非皮损、正常人表皮及高分化鳞癌皮损组织作对照。结果:CA皮损组及醋酸白试验阳性CA非皮损组中,TGF-α表达强度在表皮各层中均显著强于正常人组(P<0.05),而与鳞癌组的表达无差异(P>0.05);在表皮基底层,EGFR在正常人组、鳞癌组及CA皮损、非皮损组中均有较强表达,差异无统计学意义(P>0.05),在棘层、颗粒层正常人组及CA非皮损组中EGFR的表达均明显下降,而在CA皮损棘细胞层中,EGFR仍有较强表达(P<0.05),但弱于鳞癌组(P<0.05)。结论:HPV感染人表皮组织后,可刺激角质形成细胞以自分泌和旁分泌方式产生大量TGF-α,并可诱导棘细胞层EGFR表达上调,两者结合后可刺激多种细胞系合成DNA,引起角质形成细胞的过度增生。

Objective： To investigate the roles of TGF-α and EGFR in pathogenesis of eandyloma aeuminatum （CA）. Methods： The expression ofTGF-α and EGFR in lesion specimens from 15 patients with CA was measured with immunohistochemistry, compared with non- lesion specimens of CA patients, skin specimens from normal persons and lesion specimens of squamous cell carcinoma （SCC）. Results： The expression level of TGF-α in CA lesion and non- lesion specimens of CA was higher than that in normal skin （P 〈 0.05）. In basal cell layer, the expression level of EGFR in all groups was strong and there was no significant differences （ P 〉 0.05） between the groups; In spinal and granular layer, the expression level of EGFR in lesion specimens of CA was higher than that in normal skin and non - lesion specimens of CA （ P 〈 0.05）, and was lower than that in lesion specimens of SCC （ P 〈0.05）. Conclusion： The results suggest that increased expression of TGF-α and EGFR in CA is associated with the hyperplasia of CA.