葡萄籽原花青素对H_2O_2损伤PC12细胞的保护作用

Protective effects of grape seed proanthocyanidins on damaged PC12 cells induced by H_2O_2

目的:检测葡萄籽原花青素对H2O2引起PC12细胞氧化损伤的保护作用及其作用机制。方法:用盐酸-香草醛法检测葡萄籽原花青素中低聚花青素的含量,并用DPPH(1,1-d iphenyl-2-p icrylhydrazyl)法检测其对自由基的清除能力,以PC12细胞为实验对象,进一步检测葡萄籽原花青素对氧化损伤的PC12细胞的保护效应。结果:低聚原花青素含量为45.16%的葡萄籽原花青素在浓度为64μg/m l时对DPPH自由基的清除率为76.69%,对H2O2损伤的PC12细胞在浓度为100μg/m l时保护率为83.1%,同时可使细胞内超氧化物歧化酶(SOD),谷胱甘肽(GSH)活性增加,细胞的脂质过氧化水平降低。结论:葡萄籽原花青素是通过清除自由基和提高细胞内源抗氧化酶来保护PC12细胞免受氧化损伤。

Objective： To investigate protective effects of grape seed proanthocyanidins （GSP） on PC12 cells injury induced by H2O2 and its mechanisms. Methods： The content of oligomeric proanthocyanidins in GSP was measured using HCL-vanillin assay and its antioxidant activities were detected by DPPH （1, 1-diphenyl-2-picrylhydrazyl） radical scavenging activities. The protective effects of GSP against oxidative neuronal injuries in PC12 cells were evaluated using MTT assay. Results： The content of oligomeric proanthocyanidins in GSP was 45.16% in the present study. The elimination rate to DPPH radicals was 76.69% at the concentration of 64μg/ml. GSP demonstrated the protection 85.1% at the concentration 100μg/ml. Meanwhile GSP could increase the activities of antioxidant, including superoxide dismutase （SOD） and glutathione （GSH）, and decrease the content of malondialdehyde （MDA）. Conclusion： GSP protected PC12 cells against H2O2-induced oxidative stress, which were mediated by scavenging free radicals and increasing endogenous antioxidants.