羧甲基茯苓多糖对小鼠T淋巴细胞分泌细胞因子的影响

Effect of Carboxycyl Pachymaran on cytokines derived from T cells in Mice

目的：探讨羧甲基茯苓多糖（CMP）对小鼠T淋巴细胞分泌IFN-γ、IL-10的影响。方法：CMP溶液给小鼠腹腔注射，10天后取外周血分离T细胞，体外将不同浓度的CMP与T细胞共培养，MTT法观察其对T细胞的毒性作用，ELISA法检测IFN-γ、IL-10的含量。结果：CMP在0～500μg／ml对小鼠T细胞无明显毒性作用，CMP能显著提高IFN-γ水平，降低IL-10含量。结论：CMP具有较好的调节Th1／Th2细胞因子分泌作用。

Objective： To observe the effect of Carboxyeyl Pachymaran （CMP） on eytokines derived from T cells in normal mice. Methods： 6mg/100ml CMP solution was made and given to mice by abdominal injection once a day, the CMP dose was 0.5ml per mice, this dose was 2.5 times as human dose, in the normal control, 0.9% NS was injected at the same volume. After 10 day＇s treatment, periphrol blood were collected to derive T cell, and cytokines IFN-γ、IL-10 were detected by ELISA method, meanwhile, CMP＇s toxity on T cells was observed by MTT method in vitro. Results： CMP on the concentration of 0～500μg/ml was no cytotoxic effect on T cells. When compared with normal control group, CMP could significantly increase the level of IFN-γ, inhibit the secretion of IL-10. Conclution： CMP could regulate the secretion of Th1/Th2 cytokines on mice, in vitro experiment showed no cytotoxicity.