摘要
OBJECTIVE To investigate whether nimesulide can suppress tumor growth and induce apoptosis in SGC-7901 gastric cancer cells and to explore the molecular mechanism involved. METHODS SGC-7901 cells were cultured in RPMI 1640 medium containing different concentrations of nimesulide （0,12.5, 50, 100, 200, 400 μmol/L）. The MTT assay, morphological observation, electron microscopy （EM）, immunohistochemical analysis and Western blot analysis were employed to investigate the effects of nimesulide on the SGC-7901 cells and to explore possible related molecular mechanisms. RESULTS Nimesulide inhibited the growth of SGC-7901 cells and elicited typical apoptotic morphologic changes. Nimesulide also decreased NF-κB and Bcl-2 expression, but increased the level of the Bax protein. The positive rate of Bcl-2 protein expression at 0, 50, 100 and 200 μmol/L of nimesulide was 58.3±14.0%, 50.2±9.9%, 32.8±5.0% and 22.7±5.5% respectively based on immunohistochemical staining. The positive rate of Bax protein expression was 22.0±5.7%, 29.2±6.5%, 42.7±5.9% and 74.5±9.1% and the NF-κB expression was 74.2±10.9%, 61.8±7.6%, 36.7±10.9% and 17.5±12.3%, Significant differences were found between so μmol/L and 100 μmol/L and 200μmol/L. Western blot analysis also showed that the expression of NF-κB was decreased. CONCLUSION Nimesulide suppresses tumor growth and induces apoptosis by inhibiting NF-κB expression, which may be related to the overexpression of Bax relative to Bcl-2 expression.
OBJECTIVE To investigate whether nimesulide can suppress tumor growth and induce apoptosis in SGC-7901 gastric cancer cells and to explore the molecular mechanism involved. METHODS SGC-7901 cells were cultured in RPMI 1640 medium con- taining different concentrations of nimesulide (0,12.5, 50, 100, 200, 400 Mmol/L). The MTT assay, morphological observation, electron microscopy (EM), immunohistochemical analysis and Western blot analysis were em- ployed to investigate the effects of nimesulide on the SGC-7901 cells and to explore possible related molecular mechanisms. RESULTS Nimesulide inhibited the growth of SGC-7901 cells and elicit- ed typical apoptotic morphologic changes. Nimesulide also decreased NF-κB and Bcl-2 expression, but increased the level of the Bax protein. The positive rate of Bcl -2 protein expression at 0, 50, 100 and 200 μmol/L of nimesulide was 58.3±14.0%, 50.2±9.9%, 32.8±5.0% and 22.7± 5.5% respectively based on immunohistochemical staining. The positive rate of Bax protein expression was 22.0 ±5.7%, 29.2 ±6.5%, 42.7 ±5.9% and 74.5±9.1% and the NF-κB expression was 74.2+10.9%, 61.8±7.6%, 36.7 ±10.9% and 17.5 ±12.3%, Significant differences were found be- tween 0 μmol/L and 100 μmoI/L and 200 μmol/L. Western blot analysis al- so showed that the expression of NF-κB was decreased. CONCLUSION Nimesulide suppresses tumor growth and induces apop- tosis by inhibiting NF-κB expression, which may be related to the over- expression of Bax relative to Bcl-2 expression.
基金
This work was supported by the grant form the Natural Science Foundation of the Education Department of Jiangsu Province of China (No. 05KJD320234 and 01KJB320011).
