重症急性胰腺炎大鼠DDFA对肺损伤细胞凋亡及Bax,Bcl-2表达的影响

Effects of DDFA on cell apoptosis and expressions of Bax and Bcl-2 in lung tissue of rats with severe acute pancreatitis

目的:探讨DDFA对重症急性胰腺炎(SAP)大鼠肺组织内细胞凋亡及Bax,Bcl-2基因表达的影响.方法:大鼠45只随机分为对照组(CG),SAP组和DDFA治疗组,每组15只.大鼠SAP模型采用分2次ip200g/L-精氨酸溶液方法建立,建模后24,48和72h时测定血清TNF-a,淀粉酶.血钙及光镜观察肺组织病理变化,细胞凋亡原位检测(TUNEL)法测定肺组织内细胞凋亡,SABC免疫组化染色法测定肺组织Bax,Bcl-2基因表达.结果:SAP组血清淀粉酶,TNF-a和肺组织内细胞凋亡指数,Bax,Bcl-2基因表达较CG组升高,光镜下见肺组织损害明显;经DDFA治疗后,血清淀粉酶,TNF-a和肺组织内细胞凋亡指数,Bax基因表达下降,而Bcl-2基因表达增强,光镜下见肺组织损害减轻.结论:肺组织细胞凋亡,Bax,Bcl-2基因表达参与SAP发病机制,在SAP早期给予DDFA治疗对减轻肺脏损害是有益的.

AIM： To explore the effects of DDFA on cell apoptosis and expressions of Bax and Bcl-2 in lung tissue of rats with severe acute pancreatitis （SAP）. METHODS： 45 rats weighting 200-250 g were randomized into 3 groups ： control group （n=15）, SAP group （n=15） and DDFA group （n=15）. The models of SAP were established by the injection of 200 g/L argi- nine solution ip（ once a hour for 2 h） into the rats. At the 24, 48 and 72 h after establishment of models, serum amylase, TNF-α and calcium were determined. The left lungs were taken for light and electron microscopic observation. Cell apoptosis in lung tissue was determined by TUNEL method. Expressions of Bax and Bcl-2 were detected by immunohistochemical staining of SABC. RESULTS： In the SAP group, serum amylase, TNF-α, apoptotic index, expressions of Bax and Bcl-2 markedly increased. Lung tissue injuries were significant under a light microscope. As compared with SAP group at the same phase, serum amylase, TNF-α, apoptotic index and expressions of Bax in DDFA group decreased significantly. While the expression of Bcl-2 increased significantly. The injury of lung tissue was relieved by DDFA. CONCLUSION： The apoptosis and the expressions of Bax and Bcl-2 in lung tissue might be involved in pathogenesis of SAP. DDFA administration in the early stage is helpful for diminishing lung injury induced by SAP.