除虫菊细胞悬浮体系营养条件优化研究

Optimization on Culture Conditions in Cell Suspensions of Pyrethrum Cinerariifolium Trev

采用均匀设计和中心组合设计方法,对除虫菊细胞悬浮体系营养条件进行优化,筛选得到了除虫菊细胞悬浮培养的较优培养基,该培养基中碳、氮和磷等元素的浓度分别为40.60 g/L、85.95 mmol/L和0.78mmol/L;采用该培养基进行除虫菊细胞培养,细胞鲜重较优化前提高93%。

In order to optimize the culture conditions in cell Trev. , uniform design and central combination desig um components. The best paramenters were defined L. phosphor 0.78 mmol/L. are suspensions of Pyrethrum cinerarii f olium employed to test the improvement of medisaccharrose 40.60 g/L, nitrogen 85.95 mmol/L.