罗丹明6G分子印迹聚合物的合成与性能研究

Studies on the MoLecular Recognition and Binding Characteristics in Rhodamine 6G MoLecularly Imprinted Polymer

采用分子印迹技术,以丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,合成出以罗丹明6G为模板的分子印迹聚合物.利用静态平衡结合法和Scatchard分析法研究了此印迹聚合物的结合能力和选择性.结果表明,以丙烯酰胺为功能单体的印迹聚合物中形成了两类不同的结合位点,计算了离解常数分别为7.64ⅹ10-4moL/L和4.35ⅹ10-3moL/L.对比罗丹明S和罗丹明B的结合特性,该印迹聚合物呈现了较好的选择性.

A rnoLecularly imprinted polymer was prepared by rnoLecular imprinting technique using acrylamide as monomer, rhodamine 6G as template rnoLecule and a large excess of ethyleneglycol dimethacrylate as the cross - linking agent. The bulk polymer obtained was investigated in equilibrium binding experiments to evaluate the moLecular recognition and binding characteristics of the rhodamine 6G moLecularly imprinted polymer. Scatchard analysis showed that two sites were produced in the polymer matrix and their dissociation constants were calculated to be 7.64 × 10- ^4moL/L and 4.35 × 10^- 3moL/L, respectively. The substrate selectivity of imprinted polymer and non - imprinted polymer was investigated. The results showed that the imprinted polymer exhibited much higher affinity for rhodamine B among the tested compounds.