分子佐剂PHA增强小鼠特异性体液免疫的效应

The Molecular Adjuvant PHA Increases the Effect of Specific Humoral Immunity

目的:探讨PHA对小鼠特异性体液免疫的作用和应用价值.方法:经SRBC腹腔免疫昆明种小鼠30只,并随机等量分两组,实验组每只腹腔注射PHA 0.1ug/g,对照组注入等量N.S.5d后,两组均眼球取血,并颈椎离断处死取脾脏制备脾细胞悬液.用体外抗体形成细胞实验.(Plaque forming cell,PFC)和定量溶血分光光度测定法(Quantitativehemolysisspectrophotomentry,QHS),分别测取两组的抗体形成细胞率和QHS(495nm)OD值,经X±SD组间比较的t检验,观察有无差异性.结果:实验组的PFC形成率和QHS的OD值均显著高于对照组(p<0.01).结论:PHA有增强小鼠PFC的形成率和特异性抗体的表达.提示:PHA为分子佐剂在提高体液免疫应答的应用有较高参考价值.

Objective： To explore the function and Profitable value to humoral immunity by usingPhytohemagglatinin（PHA） to mouse. Methods： 30 kunming mice that had injected SRBC in abdomen, devided into two groups, The testing was injected PHA 0. 1ug/g in abdomen, and control group was injected N. S with equal volune. The two groups were got blood at eye and was killed to get dilutional liquid of spleen celles after five days , To ues techniques of plaque forming cell（PFC） and Quantitative hemolysis spectrophotometry（QHS） tests the PFC rate and QHS value in two groups, significant difference was found between two groups. Results ：The PFC and QHS of test group were more higher than that of eontool group（p 〈 0.01 ）. Conclusions： The molecular adjuvant PHA can promote the secretion of specific antibodies, PHA as a molecular adjuvant is worth to apply in humoral immunity response.