孕酮与A23187诱导顶体反应的研究

Study of progesterone-and A23187-induced acrosome reaction

目的:研究孕酮与钙离子载体A23187诱导顶体反应(Acrosom e reaction,AR)特征。方法:10例健康生育男性精液,采用Percoll密度梯度分离法优选精子,采用FITC-PSA荧光染色法分析AR。结果:A23187诱导AR类型主要为III型和IV型,孕酮诱导AR类型主要为II型。精子获能4、6、8和16 h时,A23187和孕酮诱导AR率与获能0 h组相比较,均明显增加,两组比较差异均有显著性(P<0.05),同一获能时间A 23187和孕酮诱导的AR率比较,差异均无显著性(P>0.05)。A23187诱导15 m in、30 m in和60 m in时,AR率均明显高于诱导前AR率(P<0.005)。孕酮诱导15 m in时,AR率与诱导前组比较没有明显差异(P>0.05),孕酮诱导30 m in和60 m in时,AR率均明显高于诱导前组(P<0.005),同一诱导时间,A23187诱导AR率均明显高于孕酮诱导的AR率,差异均有显著性(P<0.05)。结论:孕酮可诱导精子发生AR,诱导AR类型主要为II型,可比A23187诱导的AR更接近生理过程。

Objective： To investigate the character of progesterone-induced acrosome reaction and calcium ionophore A23187-induced aerosome reaction （AR） . Methods： Human semen was obtained from 10 fertile donors with normal semen parameters. Specimens were allowed to liquefy at 37℃, and were prepared by Pereoll-gradient treatment, . Spermatozoa were recovered from the upper layer. AR was analyzed using FITC-PSA staining. Results： Progesterone-induced AR mainly belongs to type Ⅱ. A23187 -induced AR mainly belongs to type Ⅲ or Ⅳ. When capacitation was achieved for 4, 6, 8 andl6 h, the percentage of progesterone - and A23187 -induced AR are both higher than that of capacitation for 0h （P 〈 0. 05 ） . When capacitation was the same time, there is no difference between progesterone - and A 23187 - induced AR （P 〉0.05 ） . When the time of A 23187 - induced AR were 15, 30 and 60 min, the percentage of AR were significantly higher than that before induced （ P 〈 0. 005 ） . When the time of progesterone-induced AR was 15 min, there is no difference between after and before induced （P 〉 0. 05 ） ; when 30 and 60 min, the percentage of AR were significantly higher than that before induced （ P 〈 0. 005 ） . In the same induced time, the percentage of A23187 - induced AR is higher than that of progesterone-induced AR （ P 〈 0. 05 ） . Conclusion ： Progesterone can induce AR, which mostly belongs to Ⅱ type and can resemble physiological process.