不同针刺频度对致敏豚鼠血清总IgE影响的实验研究

Effect of Acupuncture on Serum IgE Level in Guinea Pigs with Asthma

目的:对针刺“大椎”治疗哮喘的时效性进行初步探讨。方法:将豚鼠随机分为空白组、模型组、每日针刺组、隔日针刺组和隔2日针刺组共5组,除空白组外均用卵清白蛋白致敏并激发哮喘。选“大椎”作为治疗穴位,以放免法检测各组血清总IgE。结果:各针刺组均可使致敏豚鼠升高的血清总IgE含量下降,但下降幅度有所差异,以隔日针刺组最为明显,与模型组相比有极其显著差异(P<0·001),其次为隔2日针刺组(P<0·01),再次为每日针刺组(P<0·05)。结论:针刺“大椎”穴使血清IgE含量明显下降,提示针刺“大椎”穴对支气管哮喘有治疗作用,且随治疗频度的不同,其疗效可能不同。

Objective： To observe the effect of acupuncture of ＂Dazhui＂ （GV 14） at different frequencies on serum IgE in guinea pigs with asthma. Methods： A total of 40 guinea pigs were randomly divided into control, model, 1 d-acupuncture （once everyday）, 2 d-acupuncture （once every other day） and 3 d-acupuncture （once every 3 days） groups, with 8 cases in each group. Asthma model was established by intraperitoneal injection of 10% Ovalbumin suspension, followed by inhaling the nebulized 1% Ovalbumin plus normal saline for 30 sec. ＂Dazhui＂ （GV 14） was punctured with a filiform needle, with the needle retained for 30 min, once every day, every other day and every 3 days respectively in the 3 acupuncture groups. Serum IgE was determinecl by radioilmmunoassay. Results： In comparison with control group, serum IgE level in model group increased significantly （ P〈 0.01 ）, while compared with model group, IgE levels of the 3 acupuncture groups decreased considerably （ P〈 0.05, 0.01, 0.001 ）, and that of 2 d-acupuncture group was significantly lower than that of 1 d-acupuncture group （P〈 0.05）. No significant difference was found between ｜ d-acupuncture and 3 d-acupuncture groups （P 〉 0.05） in serum IgE content. Conclusion; Acupuncture of GV-14 can significantly lower serum IgE in asthma rats, which may contribute to its effect in relieving bronchial asthma.