超微细菌Glaciecola多糖的超声提取工艺研究

Ultrasonic treatment technology for extraction of polysaccharide from ultramicro-bacteria Glaciecola

多糖已作为免疫药物，应用于心血管疾病的临床治疗。研究了超微细菌Glaciecola多糖适宜的提取方法。通过研究常规水浸提法中不同料液比、提取温度和时间对细菌多糖得率、黏度和表面张力的影响，以及超声提取对多糖得率、黏度和表面张力的影响，结果表明；提取时间对多糖得率和多糖黏度均存在着显著的影响（P〈0．05），细菌多糖的适宜提取条件为料水比1：15，提取温度在100℃左右，提取时间4～6h。超声强化处理能显著提高超微细菌多糖的得率，降低提取液的表面张力（P〈0．05），超声强化处理后提取2h的多糖得率，可达未处理前提取6h的水平；超声提取条件以选择10～15min、100W功率，提取4h为宜。超徽细菌Glaciecola多糖的得率可达8．82％。

Polysaccharides have been used as a a chemoimmunotherapy agent in clinic treatment of heart and blood diseases. The extraction conditions of polysaccharide from ultramicro-bacteria Glaciecola were studied. Effects of sample to water ratio, extracting temperature and extracting time on polysaccharide yield, mucosity and surface tension were observed in normal water extraction and ultrasonic treatment. The results showed that there were significant differences in polysaccharide yield and mucosity among groups of different extracting time （p 〈 0.05）. The optimum extraction conditions were as follows. 1： 15 ratio of sample to water （w.v）, 100℃ extracting temperature and 4-6 h extracting period. Ultrasonic treatment was proved to be an efficient method to enhance the rate of polysaccharide yield and decrease the surface tension of solution （p 〈 0.05）. The yield by ultrasonic treatment for 2 h extracting period is the same as that by un-ultrasonic treatment for 6 h extracting period. 100 W ultrasonic power for 10-15 min and 4 h extracting period would be suggested. The polysaccharide yield from ultramicro-bacteria Glaciecola can reach 8.82%.