丹酚酸B对PDGF及MDA诱导的大鼠原代肝星状细胞增殖的影响

Effect of Salvianolic Acid B on Proliferation of PDGF and MDA-stimulated Rat Primary Cultured Hepatic Stellate Cell

目的:探讨丹酚酸B(SAB)对血小板生长因子(PDGF)和丙二醛(MDA)刺激的大鼠原代肝星状细胞(HSC)增殖的影响。方法:采用原位灌注法消化大鼠肝脏,108g/LNycodenz密度梯度离心,分离HSC,以MTT法观察细胞的增殖能力,免疫组化法检测血小板生长因子受体(PDGFR)含量。结果:MDA组与正常组相比可明显增加MTT吸光度(P<0.05),PDGF组亦较正常组明显增加MTT吸光度(P<0.01);1μmol/LSAB和10μmol/LSAB不仅可显著抑制MDA刺激的HSC吸光度增加(P均<0.01),也可抑制PDGF-BB刺激的HSC吸光度增加(P均<0.01)。PDGF及MDA作用后细胞PDGFR的表达均明显增加,而10μmol/LSAB则可抑制PDGFR的表达。结论:SAB可通过抑制PDGFR的表达而抑制体外培养HSC的增殖,这种抑制作用与SAB的抗氧化作用有一定的关系。

Objective： To research the effects of salvianolic acid B （SAB） on proliferation of PDGF and MDA-stimulated rat primary cultured hepatic stellate cells, Methods： Hepatic stellate cells were derived from normal rats by in situ perfusion, digestion and density gradient centrifugation with 108 g/L Nycodenz. The cells were stimulated by various concentrations of PDGF or MDA, and then treated with SAB. The proliferation of cells was assayed by MTF incorporation; PDGF receptor was analyzed by immunohistology. Results： MDA and PDGF obviously promoted MTI＂ incorporation compared with that of the normal cells （P〈0.05,P〈0.01）; 1 μmol/L （P〈0,01） and 10 μmol/L SAB （P〈0.01） both markedly inhibited MDA-stimulated HSC from MTF incorporation, and both 1 μ mol/L （P 〈 0.01 ） and 10 μ mol/L SAB （P 〈 0.01 ） markedly suppressed MTT incorporation of PDGF-stimulated HSC. Either PDGF or MDA induced the expression of PDGF receptor, which was decreased by 10 μmol/L SAB. Conclusion： SAB can inhibit the proliferation of rat primary cultured hepatic stellate cells by suppressing the expression of PDGF receptor, which is associated with the antioxidant effect of SAB.