摘要
目的:探讨丹酚酸B(SAB)对血小板生长因子(PDGF)和丙二醛(MDA)刺激的大鼠原代肝星状细胞(HSC)增殖的影响。方法:采用原位灌注法消化大鼠肝脏,108g/LNycodenz密度梯度离心,分离HSC,以MTT法观察细胞的增殖能力,免疫组化法检测血小板生长因子受体(PDGFR)含量。结果:MDA组与正常组相比可明显增加MTT吸光度(P<0.05),PDGF组亦较正常组明显增加MTT吸光度(P<0.01);1μmol/LSAB和10μmol/LSAB不仅可显著抑制MDA刺激的HSC吸光度增加(P均<0.01),也可抑制PDGF-BB刺激的HSC吸光度增加(P均<0.01)。PDGF及MDA作用后细胞PDGFR的表达均明显增加,而10μmol/LSAB则可抑制PDGFR的表达。结论:SAB可通过抑制PDGFR的表达而抑制体外培养HSC的增殖,这种抑制作用与SAB的抗氧化作用有一定的关系。
Objective: To research the effects of salvianolic acid B (SAB) on proliferation of PDGF and MDA-stimulated rat primary cultured hepatic stellate cells, Methods: Hepatic stellate cells were derived from normal rats by in situ perfusion, digestion and density gradient centrifugation with 108 g/L Nycodenz. The cells were stimulated by various concentrations of PDGF or MDA, and then treated with SAB. The proliferation of cells was assayed by MTF incorporation; PDGF receptor was analyzed by immunohistology. Results: MDA and PDGF obviously promoted MTI" incorporation compared with that of the normal cells (P〈0.05,P〈0.01); 1 μmol/L (P〈0,01) and 10 μmol/L SAB (P〈0.01) both markedly inhibited MDA-stimulated HSC from MTF incorporation, and both 1 μ mol/L (P 〈 0.01 ) and 10 μ mol/L SAB (P 〈 0.01 ) markedly suppressed MTT incorporation of PDGF-stimulated HSC. Either PDGF or MDA induced the expression of PDGF receptor, which was decreased by 10 μmol/L SAB. Conclusion: SAB can inhibit the proliferation of rat primary cultured hepatic stellate cells by suppressing the expression of PDGF receptor, which is associated with the antioxidant effect of SAB.
出处
《上海中医药大学学报》
CAS
2006年第2期35-36,共2页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
国家自然科学基金青年基金资助项目(30000230)
关键词
丹酚酸B
肝星状细胞
增殖周期
丙二醛
血小板生长因子
Salvianolic acid B
hepatic stellate cell
proliferation cycle
malonaldehyde
platelet-derived growth factor