应用PCR—限制性内切酶技术快速检测副结核分枝杆菌

Apply PCR Technology to Detect Mycobacterium Paratuberculosis

根据副结核分枝杆菌的IS900序列,设计相应的引物,建立检测副结核分枝杆菌的PCR技术。利用该方法,可从副结核分枝杆菌中扩增出PCR产物,扩增产物具有高度特异性,长度为388bp,经限制性内切酶Saμ3AI酶切,证实该扩增产物为副结核分枝杆菌的片段。表明此项技术具有快速、敏感和特异性强等特点,通过PCR扩增IS900基因(副结核分枝杆菌独有的基因),可快速的应用于诊断反刍动物的副结核病和乳及乳制品中副结核分枝杆菌的鉴定。

In accordance with IS900 sequence, designed homologous primer, erected to detect mycobacterium paratuberculosis PCR technology, avail the method, approving from mycobacterium paratuberculosis can amplification out PCR offspring, and possess height specificity, length for 388bp, by restrict Saμ3AI enzyme cut, confirming the amplification offspring fragment is from mycobacterium paratuberculosis. Both PCR sensibility, idiosyncratic experiment detection mycobacterium paratuberculosis show the technology possess quickly, sensitivity and specificity strong feature,Via the medium of PCR amplification is900 gene（mycobacterium paratuberculosis particular gene）, can fast apply to diagnose ruminant johnes disease, milk and dairy products for mycobacterium paratuberculosis identification.