活性氧对低氧诱导因子的调节

Reactive oxygen species in regulation of hypoxia-inducible factor

需氧生物的生存离不开氧的供应。如果在物质氧化产生能量过程中,氧未被完全还原成水,可导致氧自由基的产生,氧自由基也可转变成一些非自由基氧活性物质。由于功能上的相似性,这些物质被统称为活性氧(ROS)。ROS增多可造成蛋白质、DNA和脂质损伤。许多证据表明,氧分压改变、激素、细胞因子和化学物质等均可引起的ROS增加,作为细胞信号分子,ROS可参与细胞功能的调节。低氧诱导因子(HIF)是各种低氧诱导基因调节的关键转录因子,它的活性复合体是由2个亚单位组成的异构体。最近研究发现,无论是缺氧或非缺氧情况,ROS均参与HIF活性的调节。本文主要就ROS的产生及其对HIF活性调节方面的文献进行综述。

Oxygen is a mandatory for all aerobic organisms. Oxygen-containing free radicals are produced when oxygen is not completely reduced to water in energy-producing oxidation reaction. The radicals may also transform into other reactive compounds through electron transfer and all the compounds with similar functions are referred as reactive oxygen species （ROS）. Increased ROS is known to cause damage to proteins, DNA and lipids. Much evidence showed that changes in partial oxygen pressure, hormone, cytokine and chemical stimulation could increase ROS, and ROS, acting as signaling molecules, mediates cell functions. Hypoxia-inducing factor （HIF）, a key transcriptional factor for most hypoxia-inducible genes, is a heterodimer consisting of 2 subunits. Recent study found that ROS plays an important role in HIF activity regulation under hypoxic and non-hypoxic conditions. This paper reviews the production of ROS and its role in the regulation of HIF activity.