单侧输尿管结扎致大鼠肾间质纤维化模型中CTGF、α-SMA的动态观察

A Dynamic Observation on the Effects of CTGF and α-SMA in Renal Tubulointerstitial Fibrosis of Rats

目的：对肾脏结缔组织生长因子（CTGF）、α平滑肌肌动蛋白（α-SMA）在单侧输尿管结扎致大鼠肾间质纤维化模型中的表达进行动态观察，以探讨CTGF在肾间质纤维化病程中的作用及意义。方法：采用单侧输尿管结扎术（UUO）致大鼠肾间质纤维化模型，分别于术后第1、3、5、7、9、12天处死大鼠，HE、Mmson染色观察病理改变并进行小管间质损伤指数评分，免疫组织化学方法观察各组大鼠CTGF、α-SMA的表达。结果：第1～9天大鼠肾间质纤维化病理改变逐渐明显，CTGF、α-SMA表达逐渐增多（各组间比较，P〈0．05）；CTGF与肾小管间质损伤指数、甜SMA为正相关关系。结论：CTGF在肾小管间质纤维化的病程进展中起重要的作用，并可作为评价肾纤维化病变程度的良好指标及防治的新的靶目标。

Objective： To continuously observe the expression of CTGF and α-SMA in renal tubulointerstitial fibrosis of rats, and to investigate the significance of fibrogenic effects of CTGF. Methods： Unilateral ureteral obstruction （UUO） was adopted to produce rat models of renal tubulointerstitial fibrosis, and the rats were executed on the 1^st, 3^rd, 5^th, 7^th, 9^th and 12^th days post-operation respectively. The expression of CTGF,α-SMA in the obstructed kidney were measured by immunohistochemistry. At the same time, the degree of tubulointerstitial damage was scored. Results： The levels of CTGF, α-SMA increased gradually from the first day to the 9th day （P 〈 0.01 ） and paralleled the degree of tubulointerstitial damage. CTGF had positive correlation with the indexes of tubulointerstitial damage and α-SMA. Conclusions： CTGF may play an important role in the development of tubulointerstitial fibrosis and become a new cure target for the prevention of the disease.