摘要
[目的]在体外成功分离培养骨髓间充质干细胞(hMSCs)和测得理想冲击波(ESW)干预能量的基础上,观察体外冲击波干预后成骨活性因子IGFⅠ和TGFβ1表达,探讨其促进成骨作用。[方法]将5kV、100频次的体外冲击波作用于第1代骨髓间充质干细胞,采用免疫细胞化学法隔代观察(P1P11)胰岛素样生长因子Ⅰ(IGFⅠ)和转化生长因子β1(TGFβ1)的表达,计数阳性率;并进行统计学分析,设定P<0.05为有统计学差异。[结果]ESW干预后的第3代细胞IGFⅠ染色,胞浆及胞核中出现大量黄色棕黄色颗粒,对照组无明显着色;第7代干预组IGFⅠ呈强阳性(87.9%);TGFβ1出现较晚,第9代细胞TGFβ1阳性率为72.2%,随后开始下降;与对照组差异显著(P<0.01)。[结论]ESW干预能够提高hMSCs增殖活性,IGFⅠ和TGFβ1等的不同时期和不同程度表达是体外冲击波促进hMSCs成骨活动的适时信号和有力证据。
[ Objective] To demonstrate mechanism of ESW in curing osteogetic disorders, we studied expression of some osteogenetic factors in human mesenchymal stem cells （hMSCs） when exposed to ESW. [ Method ] After success in marrow aspiration, isolation and obtainment optimal experimental energy, a dose of 5kV and 100 times of ESW was applied to hMSCs of passage 1. The expression of IGF- Ⅰ and TGF-β1 were examined by immunocytochemical staining. [ Result] The cytochemical staining results showed that expression of IGF- I and TGF-β1 appeared at different passage of hMSCs after ESW intervention. Appearance of IGF- Ⅰ was earlier than TGF-β1 which didn＇t express until passage 7. At the same interval, the expression of IGF- Ⅰ and TGF-β1 in control group difference is lower than ESW group, respectively （P 〈 0. 01 ） . [ Conclusion] ESW of 5kV and 100 times can improve hMSCs＇ proliferation, which is associated with the appearance of osteogenetic factors, such as IGF- Ⅰ and TGF-β1.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2006年第13期1009-1012,共4页
Orthopedic Journal of China
基金
国家自然科学基金资助项目(No.30371430)
首都医学发展科研基金资助项目(No.2002-3037)
关键词
体外冲击波
骨髓间充质干细胞
胰岛素样生长因子Ⅰ
转化生长因子Β1
Extracorporeal Shock Wave （ESW）
Factor- Ⅰ（ IGF- Ⅰ ）
Transforming Growth Factor-β1 Human mesencbymal stem cells （hMSCs）, Insulin-like growth （ TGF-β1 ）
