利用流式细胞仪研究温度对两种藻竞争的影响

Influences of temperatures on the competition between two species of algae assayed by flow cytometry

为研究温度对微囊藻优势形成的影响,本试验利用流式细胞仪研究了不同温度时铜绿微囊藻(Microcystis aerugi- nosa)与蛋白核小球藻(Chlorella pyrenoidosa)间的竞争关系,在单独培养和混合培养条件下分别对两种藻细胞密度比值和细胞内叶绿素a(Chla)荧光强度进行了测定．结果表明在本试验条件下,随着温度的升高(18℃,25℃和32℃),混合培养组中微囊藻优势逐渐明显,小球藻Chla荧光强度随着温度的升高而升高,微囊藻Chla荧光强度随着温度的升高却有所降低．说明在光照适宜、高营养盐的水体中,较高的温度对铜绿微囊藻成为优势种具有明显的促进作用,进而影响着微囊藻水华的形成．

To detect the influences of different temperatures on Microcystis aeruginosa dominance, this experiment researched competition between Microcystis aeruginosa and Chlorella pyrenoidosa under different temperatures by flow cytometry, cell density ratio and chlorophyll-a fluorescence intensity in two species of algae cell were measured. Results displayed under this experimental condition Microcystis aeruginosa dominance became more evident together with temperature rised（ 18℃ ,25℃ and 32℃）. Higher temperature enhanced chlorophyll-a fluorescence intensity in Chlorella pyrenoidosa cell but reduced chlorophyll-a fluorescence intensity in Microcystis aeruginosa cell. This experiment indicated under suitable light and high nutrition salt condition, higher temperature promoted Microcystis aeruginosa dominance obviously and influenced water bloom forming.