NOS在分泌性中耳炎所致感音神经性聋中的作用

Effect of nitric oxide and nitric oxide synthase on sensorineural hearing loss secondary to secretory otitis media

目的:探讨NOS及NO在分泌性中耳炎(SOM)所致感音神经性聋(SNHL)中的作用。方法:治疗前分别抽取16例伴有SNHL的SOM患者(A组)及16例不伴有SNHL的SOM患者(B组)的中耳积液,检测两组中耳积液内NOS活性和NO含量。结果:A组中耳积液内NO含量(181.19±44.31)μmol/L明显高于B组(137.00±40.67)μmol/L(P<0.01),A组中耳积液内NOS活性(97.69±29.62)U也明显高于B组(75.50±26.99)U(P<0.05)。结论:NO可能通过直接损伤和与其他炎症递质共同作用增强局部炎症反应,在SOM所致的SNHL过程中发挥重要作用。

Objective：To evaluate the effect of nitric oxide synthase（NOS） and nitric oxide（NO） on sensorineural hearing Ioss（SNHL） secondary to secretory otitis media（SOM）. Method： Samples of middle ear effusion were collected at the time of tympanocentesis from 16 cases of SOM with SNHL（group A） and 16 cases of SOM without SNHL（group B）. And the concentration of NO and NOS activity in middle ear effusion were detected by colorimetric assay （Griess method）. Result： The concentration of NO in group A（181.19 5= 44.31）μmol/L was significantly higher than that in group B（137. 005=40.67）μmol/L（ P 〈0.01）. The NOS activity in group A（97. 695= 29.62）U was also significantly higher than that in group B（75. 505=26.99）U（ P 〈0.05）. Conclusion：Excess generation of NO in middle ear effusion which synthesized by NOS may hurt inner ear directly by its cytotoxic action or enhance local inflammatory reaction with other inflammatory factors. This study suggests that NO play a significant role in the process and pathogenesis of SNHL secondary to SOM.