银屑病患者外周血单个核细胞端粒酶活性及与疾病严重程度的关系

Relationshop Between Telomerase Activity in PBMC of Psoriatic Patients and Disease Severity

目的探讨银屑病患者外周血单个核细胞(PBMC)端粒酶活性及与疾病严重程度和病程的关系。方法分离银屑病患者和正常人外周血单个核细胞,采用聚合酶链反应-酶联免疫吸附(PCR-ELISA)法检测寻常型银屑病患者和正常人外周血单个核细胞的端粒酶活性水平;用银屑病皮损严重程度指数(PASI)评价银屑病患者病情严重程度。结果银屑病组PBMC的端粒酶活性(0.9±0.18)较正常组(0.49±0.11)显著升高(t=8.096,P<0.01),进行期(0.97±0.19)高于消退期或静止期(0.90±0.17),端粒酶活性与疾病严重程度成正相关(r=0.889,P<0.001),而与病程无明显线性关系。结论银屑病患者外周血单个核细胞端粒酶活性水平升高,且与疾病严重程度成正相关,而与病程无关,可作为银屑病发病及病情判定的一个指标。

Objective To detect the telomerase activity in peripheral blood mononuclear cells （PBMC） from psoriatic patients, and explore its correlation with psoriasis area and severity index （PASI） and duration. Methods Telomerase activity in PBMC of blood obtained from a total 19 psoriatic patients and 13 normal controls were determined by PCR-ELISA method. Results The telomerase activity was significantly higher in PBMC of psoriatic patients （0.94 ± 0.18 ） than thase in normals （0.49 ± 0.11 ） （t = 8. 096, P 〈 0.001 ）. Patients with progressive psoriasis displayed higher levels of telomerase activity （0.97 ± 0.19 ） than whom wich remissive or static psoriasis （0.90 ± 0.17 ）, but without statistical significance. A correlation between telomerase activity of PBMC and PASI score（ r = 0. 889 ,P 〈0.001 ）was found ,but no correlation between telomerase activity and duration. Conclusion Telomerase activity of PBMC correlates very well with the severity of psoriasis but nou relationship duration,and may be it is a sensitive marker of disease severity.