抗黄1号花生品种血缘关系的RAPD鉴定

The Parentage Identification of Kanghuang 1 by RAPD

以花生父本(ICGV94449A)、母本(粤油169)和子代(抗黄1号)为材料,采用RAPD研究了它们之间的亲缘关系。结果发现,引物Sangon-43、Sangon-45、Sangon-243、Sangon-257、Sangon-1094、Sangon-2098在抗黄1号中共扩增出36条带,全部能在ICGV94449A和粤油169中检出,而且Sangon-43、Sangon-45和Sangon-2098等引物的电泳图谱中有父子带;引物Sangon-243的图谱中有母子带;在引物Sangon-257的图谱中则出现了减弱带,显示了抗黄1号同时具有了ICGV94449A和粤油169的某些遗传信息,表明抗黄1号为ICGV94449A和粤油169的后代。

The origin of Kanghuang 1, a peanut breed against Aspergillus flavus, was identified in this paper. 36 bands were amplified with six primers, Sangon-43, Sangon-45, Sangon-243, Sangon-257, Sangon-1094 and Sangon- 2098, with Kanghuang 1 as their template, and all these bands could be identified from the electrophoretic map of ICGV94449A and Yueyou 169 ; but some bands that only appeared in Kanghuang 1 and ICGV94449A were found in the electrophoretic maps with Sangon-43, Sangon-45 and Sangon-2098 as their primers, and some that only appeared in Kanghuang 1 and Yueyou 169 were found in the electrophoretic map with Sangon-243 as its primer; and some which were weaker in the electrophoretic map of Kanghuang 1 than in that of ICGV94449A and Yueyou 169 were found in the electrophoretic map with Sangon-257 as its primer. It was concluded that some genetic features of Kanghuang 1 were the same with ICGV94449A, and some were the same with Yueyou 169, and others had some kind of variety in band density. It was supported that Kanghuang 1 was the offspring of ICGV94449A and Yueyou 169.