反相液相色谱法测定甲氨基阿维菌素苯甲酸盐的含量

Quantitive Determination of Emamestin Benzoate Using RP-HPLC

采用C18色谱柱，以甲醇＋φ=0．1％的三乙胺水溶液为流动相，在245nm紫外波长下，对甲氨基阿维菌素苯甲酸盐进行反相高效液相色谱分析，以外标法定量．结果显示：甲氨基阿维菌素苯甲酸盐在0．01～200mg·L^-1的范围呈良好的线性关系（r=0．9998），回收率为95．09％～101．00％，变异系数为1．32％～3．14％，检出限为0．01mg·L^-1．

To establish a RP-HPLC method for the determination of Emamestin benzoate, the reverse phase RP-HPLC of Emamestin benzoate was analyzed though employing pheromenex C18 Emamestin benzoate in environments of a reverse phase column （150 mm ×3.9 mm,4 μm） and methanol plus 0. 1% triethylamine water as mobile phase, and external standard method. Measurement results show that Emamestin benzoate show good linearity in the range of 0.01 -200 mg · L^-1 in sample solution （ r = 0.9998）, recovery rate of 95.09% - 101.00%, a coefficient of variation of 1.32% to 3.14%, and a minimum determination concentration of 0.01mg · L^-1.