水稻悬浮细胞诱导生成植保素的研究

Induction of Phytoalexins(PA) Formation in Suspension-Cultured Rice Cells by Metal and Nonmetal Ions

运用化学试剂处理水稻悬浮细胞诱导生成植保素Sakuranetin（SA）和Momilactone A（MA）．结果表明，在悬浮培养液中添加1．5mmoL／L的金属阳离子Ag^＋、Ca^2＋、Zn^2＋、Mg^2＋和Cu^2＋，都可以在一定程度上诱导悬浮细胞产生SA或MA，其中以K^＋处理效果最好；添加1．5mmoL／L的NH；也可诱导产生MA；MA的产生量显著高于SA，每20mL悬浮液中的产量可以分别高达158．9～1337．9μg和0．16～7．6μg，说明水稻悬浮细胞中存在这些阳离子的高效防御识别系统，从而开发新型绿色环保农药是可行的．

Induction of phytoalexins （PA） formation in suspension-cultured rice cells was studied with chemical method. The results showed that the defense compounds, flavonoid phytoalexins sakuranetin （SA） and diterpenoid phytoalexin momilactone A （MA） were elicited and accumulated in the suspension-cuhured cells. Of the two PA, MA showed a remarkable induction. Metal ions of silver, potassium, calcium, sodium, zinc, magnesium and copper were added to medium with a concentration of 1.5 mmoL/L, which efficiently triggered the accumulation of MA or SA. The accumulation of MA could also be elicited efficiently by ammonium ions added to the medium at concentration of 1.5 mmoL/L. It indicated the presence of recognition systems specific for these compounds in rice cells. Every 20 mL cultured cells produced 158.9 ～ 1337.9 μg MA and 0.16 ～ 7.6 μg SA, respectively, indicating the importance of this phenomenon in the defense system of rice plant and presumption of these PA for developing new type pesticide without environmental pollution. Fig 1, Tab 1, Ref 9