三角褐指藻培养条件的选择

Optimization of culture conditions for phaeodactylum tricornutum

目的:三角褐指藻作为ToxY-PAM生物检测技术的指示生物,其生长状态和活性程度是影响ToxY-PAM生物检测技术检测结果的关键。为了确保试验用的三角褐指藻处于最佳活性状态,对三角褐指藻生长的主要影响因子光照、温度和氮、磷浓度进行优化选择实验。方法:采用血球计数板计数法测定生物量,通过比较不同生长条件下藻液的相对生长速率进行优化选择。结果:三角褐指藻的最佳培养条件是:2000Lux光照强度、温度20℃、培养液12 mg/L氮和1.3 mg/L磷。结论:在上述条件下培养三角褐指藻,藻液生长快、活性强,能够准确的反映水中毒性物质对光合作用的抑制作用,可以作为ToxY-PAM生物检测技术的指示生物。

Objectives： As a indicator of the bioassay technology with ToxY - PAM, the states of growth and biological activity of phaeodactylum tricomutum were key factors for the process of biological detection. To obtain the optimization of culture conditions for phaeodactylum tricomutum, the influence of light intensity, temperature, the concentration of nitrogen and phosphor in culture medium were studied. Methods： The number of cells was counted with hemacytometer and the optimal conditions of culture selecled by comparing relative growth rates under different conditions of culture. Results ：The optimal conditions of culture of phaeodactylum tricomutum were： 2000Lux in light intensity, 20℃ in temperature, 12 mg/L nitrogen and 1.3 mg/L phosphor in cul- ture medium. Conclusion： Under above optimal conditions, phaeodactylum tricomutum grew fast and had a better biological activity. Inhibition of photosynthesis of phaeodactylum tricomutum produced by toxicants in water could be shown exactly. Phaeodactylum tricomutum in these conditions could be used as the indicator of the bioassay technology with Tox Y - PAM.