吸附剂驱铅及对染铅大鼠体内微量元素的影响

Absorbent removing lead and its effect on trace elements in lead-intoxicated SD rats

目的探讨吸附剂驱铅及对染铅大鼠体内微量元素Zn、Cu及Fe的影响。方法用0.5%醋酸铅水溶液喂养SD大鼠复制铅中毒模型,染铅20d后,喂食不同剂量的活性炭混悬液,分组喂养,同时设计正常对照组只喂蒸馏水,染铅对照组染铅后改喂蒸馏水,持续进行50d。实验第20天和第50天时,分别取大鼠尾静脉血采用原子吸收光谱测定Pb、Zn、Cu和Fe含量,并在第50天后,杀死所有动物,取出各组大鼠的肝、脑、脾和肾,用酸热消化后,用原子吸收光谱测定其中的Pb、Zn、Cu和Fe含量,各组进行比较。结果第20天时各染铅组大鼠血铅含量显著增加,同时其他微量元素显著降低,第50天时不同剂量组吸附剂均显著降低血铅浓度,同时微量元素血浓度也降低,肝、脑、脾、肾中铅含量变化同血铅,其他微量元素的变化同血中其他微量元素的变化,但铜的变化不明显。结论口服吸附剂能显著降低血铅和各组织中铅的含量,但对微量元素影响也较为明显。

[Objective] To investigate the effect of absorbent on the contents of Zn, Cu, Fe in liver, brain, spleen and kidney of lead intoxicated SD rats. [Methods] SD rats were given 0.5% lead acetate solution and after 20 days were given 5 mg（kg·d）, 10 mg/（kg· d）, 20 mg/（kg·d） activated carbon suspension in respective group, normal control group were given distilled water, lead control group were given 0.5% lead acetate solution after 20 days were given distilled water, on the 20th and the 50th day take tail venous blood of rats to measure the contents of Pb, Zn, Cu, Fe by atomic absorption spectrum. All the animals were killed on the 50th day and the contents of Pb, Zn, Cu, Fe in liver, brain, spleen and kidney were measured. [Results] On 20th day, each lead group has increasing blood lead and decreasing blood levels of other trace elements. On 50th day, absorbent at different levels all decrease blood lead and blood levels of other trace elements. Lead concentration and other trace element except Cu in liver, brain, spleen change as their concentration respectively. Change of Cu level is not significant. [Conclusion] PO absorbent can notably degrade the content of lead in blood and organisms, but can obviously affect the trace elements.