已酮可可碱对大鼠全脑缺血再灌注后神经元特异性烯醇化酶的影响

Effect of pentoxifylline on neuron specific enolase of rats after global cerebral ischemia-Reperfusion

目的探讨已酮可可碱(pentoxifylline,PTX)对大鼠全脑缺血再灌注后神经元特异性烯醇化酶(neuronspecificenolase,NSE)和神经元细胞凋亡数目的影响。方法采用四血管阻塞的方法,复制出大鼠全脑缺血再灌注模型。采用酶联免疫吸附反应(enzymelinkedimmunosorbentassay,ELISA)和原位末端标记法(insituendlabelingtechnique,TUNEL)分别检测假手术组(A组)、全脑缺血再灌注组(B组)、已酮可可碱治疗组(C组)血清NSE和海马CA1区神经元细胞凋亡数目的变化。结果与假手术组比较,全脑缺血再灌注组血清NSE浓度及和神经元细胞凋亡数目明显增加(P<0.01),并随再灌注时间的延长逐渐增多。血清NSE浓度和神经元细胞凋亡数目呈明显正相关(r=0.652,P<0.01)。PTX治疗后,血清NSE浓度和神经元细胞凋亡数目明显减少(P<0.01)。结论PTX可减少血清NSE浓度和神经元细胞凋亡数目,对脑缺血再灌注损伤有治疗作用。

[ Objective ] To explore the effects of pentoxifylline （PTX） on neuron specific enolase and the number of neuron apoptosis of rats after global cerebral ischemia - reperfusion. [Methods] The model of global cerebral ischemia - reperfusion was established by four - vessel occlusion method. Using ELISA and TUNEL, the concentration of serum NSE and the number of neuron apoptosis were examined in the sham-operated group （group A）, the control group （the group after global cerebral ischemia - reperfusion treated with Saline, group B） and the group treated with Frx （the group after global cerebral ischemia - repeffusion treated with PTX, group C） separately. [Results] Compared with group A, the concentration of serum NSE and the number of neuron apoptosis in group B increased significantly （P 〈 0.01）. With the time of reperfusion prolonged, the concentration of serum NSE and the number of neuron apoptosis gradually increased （P 〈 0.01）. Compared with group B, the concentration of serum NSE and the number of neuron apoptosis in group C decreased （P〈0.01）. [Conclusion] PTX may decrease the concentration of serum NSE and the number of neuron apeptosis on rats after global cerebral ischemia - reperfusion. It has protective effects on rats after global cerebral isehemia - reperfusion.