血瘀型肝纤维化大鼠模型的建立与评价

Establishment and Evaluation of animal model of hepatic fibrosis with blood stasis syndrome in rat

目的:复制血瘀型肝纤维化大鼠病证结合动物模型。方法:采用复合多因素的造模方法,在DMN(二甲基亚硝胺)制作大鼠肝纤维化模型的同时,多次静脉给予去甲肾上腺素和小牛血清白蛋白综合造模,对模型大鼠进行血瘀证的体征观察,采用多部位微循环显微镜行肠系膜微循环检测,并进行肝脏病理组织学观察。结果:模型(血瘀组)大鼠出现明显的血瘀表现,血瘀证候积分明显高于DMN组;血瘀组大鼠肠系膜微血管均出现典型微循环障碍,肠系膜血管管径血瘀组较DMN组增粗显著(P<0.01);血瘀组红细胞聚集明显,血液流态以粒流或粒缓流为主。肝脏病理显示血瘀组纤维化改变程度较DMN组更严重,部分肝窦中可见明显扩张瘀血。结论:病证结合动物模型既具备肝纤维化的病理特征,又符合中医血瘀证的证候表现。

Objective： To establish and evaluate the experimental animal model of hepatic fibrosis with blood stasis syndrome in rat. Meclhods： A compound multi-factors method was adopted to build the model.DMN to induce hepatic fibrosis, at the same time noradrenalin and calf bovine serrm albumin given by vein many times were synthesized to build the model. Physical signs of blood stasis of the model rats were observed. The mesenteritLm microcireulation and pathologic histology of liver were observed by multiple sites microcirculation microscope.Results; Obvious blood staffs performance appeared in modal rats, accumulated scores of blood stasis symptom was significantly higher than that of DMN group. Typical microcirculation obstacle in mesenterium capillaries, gathered RBC, grain-line stream and grain stream of blood flow showed in blood stasis rats. To compare with DMN group, the mesenteritLrn blood vessel tract was significantly wider than that of blood stasis rats （P〈0.01） . In the model rats, hepatic fibrosis degree was more serious than that of DMN group, in parts of liver sinuses obvious dilation and blood stasis could be seen. Conclusion： Hepatic fibrosis with blood staffs syndrome rats has pathologic characteristic of hepatic fibrosis, and it also fits the perforrnance of blood stasis of TCM.