二草清肝汤抗大鼠免疫性肝损伤作用机制的研究

Experimental study of Ercao Qinggan Tang on immunological liver injury in rats

目的:观察中药二草清肝汤对大鼠免疫性肝损伤的防护作用并探讨其药理学机制。方法:采用卡介苗(BCG)+脂多糖(LPS)建立大鼠免疫性肝损伤模型。做肝组织病理切片观察肝组织损伤情况;采用免疫组化法检测肝组织Bax、Bcl-2的表达;ELISA法检测血清IL-12水平。结果:大鼠免疫性肝损伤时,血清IL-12水平显著升高;肝脏Bax表达明显升高,Bcl-2表达显著降低;中药组及西药(环磷酰胺)对照组大鼠Bax的表达及IL-12水平明显低于模型组(P<0.01),而Bcl-2表达明显高于模型组(P<0.01);中药组和西药组大鼠肝组织病理改变较模型组明显减轻,中药组一般情况明显好于西药组。结论:二草清肝汤能降低免疫性肝损伤大鼠的血清IL-12水平,下调肝Bax的表达,上调Bcl-2的表达,对实验性免疫性肝损伤有明显防护作用。

Objective：To observe the protection of Ercao Qinggan Tang to immunological liver injury in rats and probe the mechanism. Methods：The immunological liver injury rat model was produced by intraperitoneal injection with BCG plus LPS. Histopathology changes of liver were detected.Immunohistochemical straining was applied to detect the expresaion of Bax and Bcl-2 in liver tissue. The content of IL-12 in serum was examined by ELISA. Results： The expression of IL-12 was apparently increased in serum of model rats. The expression of Bax was obviously raised in tissue of liver while Bcl-2 was evidently decreased. Chinese medicine groups and western medicine group were all higher than the model group on the expression of Bcl-2 （P〈0.01 ） . While lower on the expression of Bax,IL-12 （P〈0.01 ） . Chinese medicine groups and western medicine group were much better than the model group on histopathologic changes.The Chinese medicine groups were apparently better than the western medicine group in general conditions.Conclusion：Ercao Qinggan Tang can reduce the expression of IL-12, Bax and increase the expression of Bel-2.The herb can obviously protect immunological injury to liver in rats.