摘要
超高效液相色谱比高效液相色谱分析速度更快更灵敏,它使用1.7μm颗粒度的色谱柱填料,能够获得更高的柱效,并且在更宽的线速度范围内柱效保持恒定,因而有利于提高流动相流速、节省分析时间和增加峰容量,这种固定相还减少了表面残余硅羟基,适合于分析中药中生物碱类等特殊样品。本论文对超高效液相色谱在复杂体系中药中的分离分析进行了简要介绍,尽管使用小粒径的色谱柱填料,超高效液相色谱本质上应符合液相色谱的保留值规律、峰形规律,我们对此进行了初步验证。研究表明了超高效液相色谱将在复杂体系中药的分离分析中发挥重要的作用。
Ultra-performance Liquid Chromatogram （UPLC） was faster and more sensitive than HPLC. Waters synthesized and developed 1.7μm are higher efficiencies full advantage of chro particles as UPLC column solid phase. A realm of chromatography is entered where not only gains, but also these efficiencies no longer diminish with flow rate. It is now possible to take matographic principles to run separations, using shorter columns and higher flow rates for increased speed, with superior resolution and sensitivity. Packings based on high-purity silicas that are well endcapped show a low silanol group activity. It shows significant benefit on the separation of alkaloids in Traditional Chinese Medicines （TCM）. In this paper the applications of UPLC in separation and analysis of TCM were introduced. The retention value （a, c） and peak shape rule in UPLC was also studied. It showed that retention value （a, c） and peak shape rule in UPLC were similar to HPLC. The results indicate that UPLC will play an important role in the separation and analysis of TCM.
出处
《世界科学技术-中医药现代化》
2006年第3期106-111,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
中国科学院知识创新工程重要方向项目(KGCX2-SW-213)基于现代理论和技术的复方中药系统研究
国家自然科学基金重点项目(20235020)中药药效组分的指纹图谱分析的方法研究
