大白菜种质资源的遗传多样性分析

Use of Random Amplified Polymorphic DNA(RAPD) Markers for the Identification of Genetic Diversity in Chinese Cabbage

采用RAPD技术对64份大白菜种质资源的遗传多样性进行了研究。从OPE,OPF,OPG和OPH 4套共80条引物中选出了16个条带清晰、且具有多态性的引物分析供试材料,结果共产生100条扩增带,其中72条带具有多态性,多态性频率平均为72%。由RAPD分析结果可初步得出芜菁、小白菜和大白菜的特征谱带,这些特征谱带可分别作为这3个亚种的RAPD标记,据此可鉴定这3个亚种。将扩增出来的72条多态性片断进行统计,采用UPGMA进行聚类分析,取相似距离0.721为阈值,将大白菜种质资源分为6个类群,而小白菜(66号,苏州青)和芜菁(67号)各成一类,共8个类群。

Genetic diversity of sixty-four germplasm accessions of Chinese cabbage （ B. pekinensis ） with a turnip （B. rapa ） and a Xiaobaicai （B. chinensis L. ） were analyzed using RAPD （Random Amplified Polymorphic DNA） marker. Sixteen primers with sharp and polymorphic bands selected from four sets of primer （OPE, OPF, OPG, OPH） that have eighty primers were selected to analyze materials. 100 amplified bands were produced. Among them, 72 bands were polymorphic. The percentage of polymorphic was 72 %. The results can show the typical bands of turnip, Xiaobaicai and Chinese cabbage. These three typical bands can be used as RAPD markers for identification of these three species. Nei-Li similarities computed by 72 polymorphic DNA markers were used in unweighted pair group method of arithmetic mean （UPGMA） cluster analyses. All materials were clustered into groups corresponding to the eight types by similarity （0.721）, i.e., six Chinese cabbage types which include 64 accessions of Chinese Cabbage, turnip type and Xiaobaicai type.