摘要
[目的]测定生首乌及制首乌总多糖的含量。[方法]采用苯酚一硫酸比色法。[结果]486nm处有最大吸收峰，线性范围在10．5～94．5μg·L^-1，平均回收率为101．23%（n=5，RSD=3．03％），生首乌中总多糖含量为14．28％，制首乌中多糖为19．03％。[结论]该方法快速简便，灵敏度高，为制剂生产的质量控制提供参考。
[Objective]To determine the content of Polygonum multiflorum thunb. Polysaccharide and the refined. [Methods] The sulfuric acid-phenol method was used. [Results] λmax= 486nm. The linear rang was 10.5-94. 5μg · L^-1. And the average recovery was 101.23%（n=5,RSD=3.03%）. The content of Polygonum muhiflorum thunb. Polysaccharide was 14.28% and the refined was 19.03%. [Conclusion] The method was simple, rapid and reliable.
出处
《浙江中医药大学学报》
CAS
2006年第4期419-420,共2页
Journal of Zhejiang Chinese Medical University
关键词
何首乌
制首乌
总多糖
苯酚-硫酸法
含量测定
Polygonum multiflorum thunb.
Refined Polygonum multiflorum thunb.
Polysaccharide
Sulfuric acid-phenol method
content determination
