安宫牛黄散中配伍药物对雄黄可溶性砷含量的影响

Effects of components in Angong Niuhuang Pulvis on solubility of arsenic in Reaglar

目的以安宫牛黄散为实例,研究中药复方中的配伍药物对雄黄中砷的溶解性的影响。方法安宫牛黄散中几种单味药与雄黄混合用人工胃液处理,用原子荧光法测定可溶性砷的含量。结果安宫牛黄散中几种单味药分别与雄黄混合用人工胃液处理后,测得的可溶性砷含量均比雄黄在人工胃液中的可溶性砷的溶出量低,使可溶性砷含量溶出减少的单味药次序依次为黄芩、黄连、珍珠、栀子、郁金和牛黄,溶出的可溶性砷含量依次减少55.1%,49.3%,29.4%,25.2%,25.0%和11.4%(P<0.01)。安宫牛黄散中的可溶性砷含量比雄黄的可溶性砷含量减少35.3%(P<0.01)。结论安宫牛黄散中的几种单味药均有抑制雄黄中可溶性砷溶出的作用,推测是降低砷的毒性作用的可能途径之一。

Objectlve To study the effects of components in Angong Niuhuang Pulvis on solubility of arsenic in Reaglar. Methods Reaglar and components in the complex prescription were pretreated with simulated gastric juice respectively, and then concentrations of solvable arsenic were determined by intermittent flow hydride generation-atomic spectrophotofluorimetry. Results Contents of solvable arsenic in simulated gastric juice-pretreated Realgar with components in Angong Niuhuang Pulvis or the complex prescription were lower than those in simulated gastric juice-pretreated Realgar. Components of Huangqin （Radix Scutellariae） , Huanglian （Rhizoma Coptidis ） , Zhenzhu （Margarita）, Zhizi （ Fructus Gardeniae ） , Yujin （Radix Curcumae ） and Niuhuang （Calculus Bovis ） decreased the contents of solvable arsenic in Realgar by 55.1% , 49.2% , 29.4% , 25.2% , 25.0% and 11.4% respectively （P 〈 0.01 ）. The content of solvable arsenic in Angong Niuhuang Pulvis is 35.3% lower than that in Reaglar （P 〈 0.01 ）. Conclusion Components in Angong Niuang Pulvis can reduce solubility of arsenic in Realgar, which may reduce toxicity of arsenic.