罗格列酮在Beagle犬体内血药浓度测定方法研究

HPLC method for the determination of rosiglitazone in serum of Beagle dogs

目的建立高效液相色谱法测定Beagle犬体内罗格列酮血药浓度的方法。方法以十八烷基键合硅胶柱为固定相．以0．01mol／L的磷酸二氢钾水溶液-乙腈（50：50），以三乙胺调节pH6．0为流动相，检测波长247nm。4-硝基-2-苯氧基甲烷磺酰苯胺为内标，样品用乙醚提取，按内标法定量。结果标准曲线50—5000ng／ml范围内有良好线性关系（r=0．9996），最低检测浓度为30ng／ml，平均方法回收率为97．34％（RSD为2．15％）。结论本文建立的方法能够准确测定罗格列酮的血药浓度，线性关系好，回收率高，稳定耐用。

Objective To establish a assay method for the determination of rosiglitazone in serum of Beagle dogs. Method Analysis was conducted with the column of C8 （250 × 4.6 mm, 5μm） and mobile phase of 0.01 mol/L potassium dihydrophosphate-acetonitrile （50： 50） （pH value was adjusted to 6.0 with triethylamine）, and rosiglitazone was determined at the wavelength of 247 nm. , and 4-nitro-2-phenoxymethane sulfonanilide as interal standard. Results In the eoneentration range of 50～5000 ng·ml^-1 , the distribution of rosiglitazone is linear （r = 0.9996） , with an average reeovery of 97.34% （RSD was 2.15% ）. Conelusion The assay method is convenient, aeeurate, and reliable with good preeision, and ean be used in the determination of rosiglitazone in serum of beagle dog.