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HPLC-ELSD测定不同产地青蒿中青蒿素的含量 被引量:4

Determination of artemisinin in Artemisia apiacea Hance from different regions by HPLC evaporative light scattering detection method
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摘要 目的建立HPLC—ELSD同时测定不同产地青蒿中青蒿素的含量。方法采用色谱柱:LichrospherC18柱(4.6mm×250mm,5μm);流动相:甲醇:水(75:25);Alltech ELSD 2000(蒸发光散射检测器)检测,气体为高纯氮气,流速2.0L/min。结果在此色谱条件下,青蒿素与其它色谱峰完全达到基线分离(R〉2.0)。12批不同产地黄花蒿中青蒿素含量在0.25%~1.21%。结论用该方法进行检测,操作简单,灵敏度高。 Objective To establish a method to quantify the artemisinin in Artemisia annuan L. from different regions. Methods Separtion was performed in a chromatographic column : Lichrosper C18 (4.6 mm × 250 mm,5μm ) , and mobile phase consisted of : Methyl alcohol:water(75:25 ) . Alltech ELSD 2000 (electronic light scattering detector)was used. Results Under this chromatographic condition the baseline totally disunited between the peak of artemisinin and other chromatographic peaks . The quantity of artemisinin in twelve batches Artemisia annua L. in different regions is between 0.25% - 1.21%. Conclusion This method is sensitivity and convenient for dertermination of artemisinin.
出处 《现代食品与药品杂志》 2006年第3期34-36,共3页 JOurnal of Modern Food and Pharmaceuticals
关键词 青蒿 青蒿素 HPLC—ELSD Artemisia apiacea Hance artemisinin HPLC-ELSD
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