加味丹参饮对麻醉犬心肌缺血及相关血液生化指标的影响

Effect of Danshen Yin on Myocardial Iischemia and Related Biochemical Index of Blood in Dogs

目的探讨加味丹参饮对麻醉犬急性心肌缺血及相关血液生化指标的影响及作用机制。方法将25条犬随机分为模型组、恬尔心组(阳性组)、加味丹参饮大、中、小剂量组。每组5只。采用麻醉犬心外膜电图标测心肌缺血程度,定量组织学(N-BT染色法)测定心肌梗死范围,股动脉抽血测定血清肌酸激酶(CK)、(LDH)的活性变化。结果在给药后30、60、90、120min,加味丹参饮大、中、剂量组∑-ST分别与模型组比较均有统计学意义(P<0.05,P<0.01),阳性组有同样作用,但与加味丹参饮组比较无统计学意义(P>0.05);对心肌梗死面积的影响,在给药后120min,加味丹参饮3个剂量组及阳性组与模型组比较均具有统计学意义(P<0.001);在给药后60、120min,加味丹参饮大、中剂量组分别与模型组血清CK、LDH的活性比较均有显著性意义(P<0.05),阳性组亦有同样的作用。结论加味丹参饮对麻醉犬具有抗心肌缺血和保护心肌细胞作用。

Objective To observe the effect of Danshen yin （DSY） on the myocardial ischemia and the change of related biochemical index of blood in dogs. Methods Myocardial ischemia models were induced by ligating anterior descending branch of the left coronary artery of the dogs. The range and degree of myocardial ischemia were detected respectively via epicardical electrocardiograph and nitroblue tetrazolium staining, and the change of CK and LDH in the plasma activities were measured. Results In both DSY groups and positive medicine group, ∑-ST in the myocardial ischemia reduced significantly compared with model group （P〈0.05,P〈0.01）, but, there was no difference between DSY groups and the positive group（P〈0.05） after giving medcine 30, 60, 90 and 120 min. The effect of DSY groups and the positive group on the range of myocardial ischemia was limited strongly after 120 min compared with the model group （P〈0.001）, and the activity of CK and LDH was lower than that in model group（P〈0.05）. Conclusion DSY has obvious protective effect on acute myocardial ischemia and the positive medicine contrast group has the same effect.