儿童哮喘过敏原皮肤点刺试验和血清总IgE及ECP检测的临床意义

Analyzing clinical significance of skin prink test,total IgE and ECP in serum in children with asthma

目的:探讨皮肤变应原点刺试验及血清总IgE、ECP的检测在儿童哮喘诊断中的临床意义。方法:选用粉尘螨、艾蒿花粉、狗毛、香烟、牛奶5种过敏原,对61例哮喘患儿及66例对照组儿童进行皮肤点刺试验。其中对50例哮喘患儿和56例对照组儿童采用ELISA法进行血清总IgE的检测,采用荧光酶联免疫法进行血清ECP检测。结果:(1)61例哮喘患儿变应原皮肤点刺试验过敏原总阳性例数为40例(66.67%)。(2)哮喘组5种过敏原皮肤点刺阳性率:粉尘螨31.15%、狗毛24.59%、艾蒿花粉39.34%、牛奶21.31%、香烟29.51%。(3)哮喘组血清总IgE高于对照组(P<0.05),但两组的血清ECP值差异无统计学意义(P>0.05)。(4)血清总IgE与血清ECP之间无相关性(r=0.284 6,P>0.05)。结论:皮肤点刺试验痛苦小,简便易行,特别适用于儿童。联合检测血清总IgE和ECP可作为临床诊治哮喘的依据。

Objective： To evaluate the clinical significance of skin prink test （SPT）, total IgE and ECP in serum of asthma in children. Method： Sixty-six children with asthma and healthy children in control group were used by SPT with dermatophagoides farinae, dog hair, cow＇s milk, cigarette and mugwort pollen, at the same time, the level of total IgE and ECP in serum of 50 children with asthma and 56 control group children were checked by ELISA and fluorescence enzyme linked-immunization. Result： （1） The total positive rate of SPT of 61 children with asthma was 66.67%. （2） Positive rate of SPT of children with asthma abowt five allergens are as follows： dermatophagoides farinae 31. 15%, dog hair 24. 59%, cow＇s milk 21.31%, cigarette 29.51%, mugwort pollen 39.34%. （3） The level of total IgE in serum in children with asthma were higher than that of control group. But the level of ECP in serum in children with asthma were not higher than that of control group. （4） There were no correlation between total IgE and ECP in serum. Conclusion： Skin prink test can be choosed to check allergen in children with asthma firstly,and companied with total IgE and ECP in serum as basis for diagnosis and therapy of asthma.