解偶联蛋白2基因多态性与糖尿病合并脑血栓的关系

Association between uncoupling protein 2 gene polymorphism and diabetic patients with cerebral thrombosis

目的探讨解偶联蛋白2(UCP-2)基因8号外显子3′-末端45bp插入/缺失多态与糖尿病合并脑血栓的关系。方法178例糖尿病患者(伴有脑血栓组49例,不伴脑血栓组129例),检测所有患者的基因组DNA,用聚合酶链反应(PCR)方法对UCP-2基因进行扩增,记录各组UCP-2等位基因及基因型频率并进行比较。结果UCP-2基因8号外显子3′-末端45bp插入/缺失多态在伴有脑血栓组和不伴脑血栓组中基因型分布差异无统计学意义(χ2=1.17,P>0.05),两组的等位基因频率差异亦无统计学意义(χ2=0.24,P>0.05)。结论UCP-2基因8号外显子3′-末端45bp插入/缺失多态与中国人糖尿病并发脑血栓无相关性。

Objective To investigate the association of uncoupling protein 2 （ UCP - 2） gene a 45 bp insertion/deletion（Ins/Del） polymorphism in the 3, -untranslated （3, -UTR） of its exon 8 on diabetic patients with cerebral thrombosis. Methods A total of 178 patients were selected 49 cases with cerebral thrombosis, 129 cases in normal control group, UCP- 2 gene polymorphism was confirmed by electrophoresis after PCR with 3% agarose, then compared genotype and allele gene frequency. Resuits The assay suggested that no significant difference of genotype frequencies for 3, - UTR Ins/Del polymorphism of UCP - 2 between control group and cerebral thrombosis group, and there was also no difference in two alleles frequencies. Conclusion No relationship of the 3, - UTR a 45 bp Ins/Del polymorphism in exon 8 of the UCP - 2 gene is found in diabetic patients with cerebral thrombosis.