模拟水解酶的合成及其催化动力学

Synthesis of mimetic hydrolase and studies of catalytic kinetics

介绍了2种具有吗啉环的Schiff碱过渡金属配合物的合成和表征,并且配体相同而金属离子不同的2种配合物作为模拟羧酸酯水解酶被用于催化对硝基吡啶甲酸酯(PNPP)水解.研究了催化反应的动力学机理,建立了PNPP催化水解的动力学模型,并计算了相关的动力学和热力学常数.研究结果表明:2种配合物在催化PNPP水解中表现出较好的催化活性;在缓冲溶液中随着溶液pH的增大,PNPP催化水解速率提高;配合物中金属离子极化作用影响PNPP催化水解速率.

It has been reported that two Schiff base transitional metal complexes with morpholine ring were synthesized and characterized, and two complexes with same agent and different metal ion were used in the catalytic hydrolysis of p - nitrophenyl picolinate acetate as a simulant hydrolase of carboxylic acid ester. Kinetics and mechanism of PNPP catalytic hydrolysis are studied. The kinetic model of PNPP catalytic hydrolysis is proposed. The kinetic and the thermodynamic constant on the catalytic reaction are calculated. The result of the study shows： two complexes revealed good catalytic activity in PNPP catalytic hydrolysis. The rate of the PNPP catalytic hydrolysis is increased following increasing pH value of the buffer solution and affected by the polarization action of metal ion of complex.