青藤碱和甲氨蝶呤对降植烷诱导的关节炎大鼠血清IL-10和TNF-α的影响

Effect of Sinomenine and MTX on Serum Levels of IL-10 and TNF-α in Rat Models with Pristane Induced Arthritis(PIA)

目的:探讨青藤碱和甲氨蝶呤对降植烷诱导的关节炎大鼠(pristane induced arthritis,PIA)血清白细胞介素-10(interleuk in-10,IL-10)和肿瘤坏死因子-α(tumor necrosis factor-,αTNF-α)的影响。方法:将40只Lew is大鼠随机平均分为5组,即正常组、模型组、青藤碱组、MTX组、青藤碱+MTX组,除正常组大鼠,在其它大鼠尾根部位皮内注射0.2m l/只剂量的降植烷以建立PIA模型,同时口服青藤碱(120mg.kg-1.d-1)和/或MTX(3.8mg.kg-1.w-1)共8w。正常对照组大鼠皮内注射等量的生理盐水并口服生理盐水(10mg/kg/w)。给药8w后采血,用放射免疫分析检测血清IL-10和TNF-α的水平。结果:MTX组大鼠血清IL-10(及TNF-α)水平均明显低于模型组大鼠(P<0.05);模型组大鼠血清TNF-α含量明显高于正常对照组和含MTX治疗组(P<0.05),与单用青藤碱治疗组比较无显著性差异。结论:甲氨蝶呤可能对PIA大鼠血清IL-10和TNF-α均有明显抑制作用。

Objective To study the effect of sinomenine and MTX on the serum levels of IL - 10 and TNF - α in rat models of PIA. Methods Thirty - two rat models of PIA were prepared with subcutaneous injection of pristane （0.2ml）. These rat models were divided into four groups （n = 8 in each group） ： Group A, on treatment given; Group B, treated with sinomenine （120mg/kg/d）, Group C, treated with MTX （3.8mg/kg/d） ; Group D, treated with sinomenine and MTX combined. Another 8 rats comprised the control group. All the 40 rats were sacrificed after 8 weeks; serum IL - 10 levels were determined with RIA and severity of arthritis （with score 1 -4） evaluated. Results Serum levels of both IL - 10 and TNF - α in the rat models treated with MTX alone were the lowest among all the animals and were significantly lower than those in the untreated models. Levels of TNF - α in the untreated models were significantly higher than those in the controls and those in the MTX -treated group （P 〈 0.05 ）, but were not significantly different from those in the models treated with sinomenine alone. Conclusion MTX demonstrated prominent inhibition over serum expression of IL - 10 and TNF - α in rat models with PIA.