PTEN-PKB途径在子宫内膜癌中的作用

Role of PI3K-PKB Pathway in Endometrial Carcinoma

目的探讨PTEN、PKB在子宫内膜癌发病过程中的作用。方法应用逆转录聚合酶链反应 (RT-PCR)技术检测55例子宫内膜癌组织,13例子宫内膜非典型增生,11例增殖期内膜中PTEN及PKB mRNA的表达。结果 PTEN mRNA在正常内膜、非典型增生内膜及子宫内膜癌组织间表达阳性率分别为 81．8％、38．5％及36．4％,三者比较有显著性差异(P<0．05),而PKB mRNA在正常内膜、非典型增生及子宫内膜癌中表达阳性率为27．3％、46．2％及61．8％,但无统计学差异(P>0．05);在子宫内膜癌不同临床分期、病理分级之间,PTEN mRNA和PKB mRNA表达量均没有显著差异(P>0．05)。结论 PTEN可能通过PI3K- PKB途径导致子宫内膜癌的发生。

Objective To investigate the role of PTEN and PKB in the occurenc of endometrial carcinoma.Methods Dectect the expression of PTEN and PKB mRNA in 52 cases with endometrial carcinoma and 11 cases with endometrial hyperplasia by RT-PCR, take 19 cases with normal endometrium as control. Results Positive rate of PTEN mRNA in normal endometrium was 81.8%, and the positive rate in endometrial hyperplasia and carcinoma was 38.5% and 36.4%. The difference between the three groups was remarkable（p〈0.05）. Positive rate of PKB mRNA in normal endometrium, endometrial hyperplasia and carcinoma was27.3%, 46.2% and 61.8%.There was no significant difference between the three groups （p〉0.05）.The positive rate of PTEN and PKB expression in different clinical stage, pathological grade and muscular invasion of endometrial carcinoma was of no difference. Conclusion PTEN can regulate PI3K-PKB pathway, leading to the onset ofendometrial carcinoma.