青光眼患者房水TGF-β_2含量变化及与滤过泡形成的关系

TGF-β_2 levels in the aqueous humor of glaucoma and the filtering bleb development

目的探讨青光眼患者房水中转化生长因子(TGF)-2β含量变化及其与小梁切除术后滤过泡形成的关系。方法手术进入前房之前采集74例88眼原发性闭角型青光眼(PACG组)、9例10眼新生血管性青光眼(NG组)、21例21眼再次手术的青光眼(SOG组)和14例16眼单纯老年白内障(正常对照组)患者的房水。应用酶联免疫吸附测定法测定其总的和活化的TGF-2β水平。并将其中初次行小梁切除术未使用丝裂霉素(MM C)的82眼做为期16个月的随访,分析房水中TGF-2β水平与滤过泡形成的关系。结果SOG组房水中总的和活化的的TGF-2β含量明显高于正常对照组和PACG组,NG组房水中总的TGF-2β含量明显高于正常对照组。PACG组房水中总的和活化的TGF-2β含量与正常对照组相比,差异统计学意义。82例未用MM C初次行小梁切除术者随访结果表明手术失败组房水中总的TGF-2β水平明显高于手术成功组,差异均有统计学意义。结论TGF-2β在青光眼术后滤过口愈合及瘢痕化过程中起重要作用,在眼部新生血管形成过程中也起到一定作用。

[Objective] To investigate TGF-β2 levels in the aqueous humor of different types of glaucoma and the relation to filtering bleb development after trabeculectomy. [Methods] Aqueous humor was collected at the beginning of surgery from 119 eyes with glaucoma （88PACG eyes, 10 NG eyes, 21 SOG eyes） and from 16 control eyes that underwent cataract operation. TGF-β2 levels （intrinsically activated and total TGF-β2 as well as total protein concentrations of the aqueous humor were determinedby using ELISA methods. 82 cases of first trabeculectong eye witheut were folloued up for 6 months. [Results] The total and intrinsically activated TGF-β2 concentration in the aqueous humor of SOG was significantly higher than that of control and PACG groups; The total TGF-β2 concentration in the aqueous humor of NG group was significantly higher than that of control group; The total and intrinsically activated TGF-β2 in the aqueoas humor of eyes with PACG was not significantlg different from that in eyes with control. A T-test analysis showed a significant difference of total TGF-β2 level between failure and successful filtering bleb formation during 6 months＇ follow-up of the 82 eyes uderwent first trabeculectomy without MMC. [Conclusion] There might be some relationship between bleb formation and TGF-β2 levels. TGF-β2 may play an important role in the eye angiogenesis.