一种基于碳纳米管的安培型过氧化氢生物传感器

A Hydrogen Peroxide Amperometric Biosensor Based on Carbon Nanotubes

利用硫堇（thionine,Thi）作为介体结合多壁碳纳米管（MWNTs）、壳聚糖（chitosan,CHIT）、辣根过氧化酶（HRP）的混合包埋物制作过氧化氢（H2O2）生物传感器.研究结果表明所得传感器对H2O2具有明显的增敏效应,线性范围为0.03～5.5 mmol/L,相关系数为0.9995;检出限为19 μmol/L（S/N=3）,具有良好的稳定性及工作寿命.

A novel hydrogen peroxide biosensor was constructed based on the characteristics of the carbon nanotubes. The matrix of the multi-walled carbon nanotubes,chitosan and horseradish peroxidase was immobilized on the surface of the glassy carbon electrode,which was modified by cationic dye- thionine as a mediator. A linear range from 30μmol/L to 5.5 mmol/L was observed and the detection limit was 19 μmol/L when signal to noise ration （S/N） was 3.This biosensor shows high sensitivity, good stability and reprodueibility.