慢性肝病网织血小板和血小板生成素的检测及临床意义

Clinical significance of measuring reticulate platelets and thrombopoietin in patients with chronic liver diseases

目的:探讨网织血小板(RP)、血小板生成素(Tpo)与慢性肝病血小板减少的关系。方法:分别用流式细胞仪和ELISA方法测定正常人群及慢性肝病患者RP和Tpo水平。结果:①与正常人群比较,慢性肝炎、肝硬化患者RP绝对计数均显著降低;肝病患者血小板正常组与减少组比较RP和RP%均有显著性差异。②与正常人群比较,慢性肝炎、肝硬化患者Tpo水平均无显著性差异;而肝病患者血小板正常组与减少组比较Tpo水平有显著性差异。结论:血小板减少的慢性肝病患者存在血小板的生成减少,而Tpo水平下降可能是血小板生成减少的一个重要原因。

Objective： To measure the percentage and absolute counts of reticulated platelet（RP） and thrombopoietin（Tpo） levels in patients with chronic liver diseases. Methods： RP and Tpo were detected with ELISA and FCM method in patients with liver diseases and healthy controls. Results： ①The RP count of patients with chronic hepatitis and liver cirrhosis（LC ） was significantly low- er than that of normal people;The RP count and RP% were significanly different in chronic liver diseases patients with normal or abnormal platelet. ②Tpo level was not significantly different among patients with chronic hepatitis, liver cirrhosis and healthy controls; Patients with normal platelet counts showed higher thrombopoietin level than those with low platelet counts. Conclusion： These findings suggest that patients with chronic liver diseases patients with thrombocytopenia present decreased platelet,and decreased Tpo level may be one important reason for decreased platelet level.