肥胖大鼠白色脂肪组织中PPAR_γ、UCP2基因与血清1_α,25-(OH)_2-D_3的相关性研究

Study on the relationships between PPAR_γ,UCP2 genes in white adipose tissue and serum 1_α,25-(OH)_2-D_3 in high-fat-induced obesity rats

目的:探讨高脂饮食诱导下肥胖大鼠白色脂肪组织(whiteadiposetissue,WAT)中过氧化物酶体增殖物激活受体γ(peroxisomeproliferator-activatedreceptor-γ,PPARγ)、解偶联蛋白2(uncouplingprotein2,UCP2)基因与血清1α,25-(OH)2-D3的相关性。方法:①36只SD大鼠,标准饲料平衡1周后,随机分成2组,肥胖组和标准对照组,分组后分别用高脂饲料和标准饲料继续饲养6周;②采用ELISA方法测定血清1α,25-(OH)2-D3;③RT-PCR技术分析WAT中PPARγ和UCP2基因mRNA的表达水平。结果:①肥胖组大鼠的体重增值高于标准对照组(P<0.05),提示肥胖模型制备成功。②肥胖大鼠血清1α,25-(OH)2-D3低于标准对照组(P<0.05)。③WAT中PPARγ和UCP2mRNA表达均高于标准对照组(P<0.05)。结论:肥胖大鼠WAT中PPARγ诱导UCP2高表达,1α,25-(OH)2-D3与UCP2mRNA表达趋势相反。

Objective： To explore the relationships between peroxisome proliferator-activated （PPARγ）,uncoupling protein 2 （UCP2） gene in white adipose tissue （WAT） and serum 1α,25-（OH）2-D3 in high-fat induced obesity rats. Methods： After 1 week of free access to a standard diet, 36 rats were randomly divided into obesity group and control group. Rats were fed on high-fat diet or standard diet respectively for 6 weeks. The serum 1α,25-（OH ）2-D3 was detected by ELISA technique, and the mRNA expression level of PPARγ and UCP2 genes in WAT were detected by RT-PCR technique. Results： ①In obesity group, the speed at which the body weight increased was higher than that in control group （P 〈 0.05） . ②The serum 1α,25-（OH）2-D3 in obesity group was lower than that in control group（P 〈 0.05）. ③The mRNA expression level of PPARγand UCP2 genes in WAT was higher in obesity goup compared with control group （P 〈 0.05）. Conclusion： In WAT of obesity rats, PPARγ gene is highly expressed and can induce UCP2 gene expression; the tendency between the mRNA expression level of UCP2 and serum 1α,25-（OH）2-D3 was reverse.