老年慢性阻塞性肺疾病缓解期T淋巴细胞值与细胞因子浓度

The Level of T Lymphocyte Subpopulation and Cytokines in Senile Patients with Stable Chronic Obstructive Pulmonary Disease

了解老年慢性阻塞性肺疾病(COPD)缓解期T淋巴细胞亚群中CD3+、CD4+、CD8+比值和血IL4、IL8及TNFα的浓度,为治疗和监测疾病的发展提供依据。对老年COPD缓解期组50例和老年对照组40名分别采用流式细胞仪测定外周血CD3+、CD4+、CD8+;应用RIA测定血清IL4、IL8、TNFα含量。COPD缓解期组CD3+、CD4+较对照组低,有显著性差异(P<0.05);IL4、TNFα明显高于对照组(P<0.05)。而CD8+、IL8在COPD缓解期组略高于对照组,但两组间无显著性差异。COPD患者CD3+、CD4+的减低和IL4及TNFα的增高显示在COPD缓解期仍存在着细胞免疫功能紊乱和持续的炎症反应。

To investigate the level of T-lymphocyte subsets（CD3^＋ , CD4^＋ , CD8^＋） and serum IL- 4,IL-8 and TNF-α in senile patients with stable chronic obstructive pulmonary disease（COPD） and provide basis in observing the inflammatory process of COPD, 50 senile patients with stable COPD and 40 senile healthy persons as control group were selected, and levels of IL-4, IL-8 and TNF-α in serum were detected by RIA method, the T-lymphocyte subset （CD3^＋ , CD4^＋ , CD8^＋）of peripheral blood was measured by flow cytometry. The levels of CD3^＋, CD4^＋ in COPD group were lower than in control group （P〈0.05） and IL-4, TNF-α were higher in COPD group than in control group（P〈0.05）. And CD8^＋ , IL-8 were also slightly higher in COPD group compared with that in control group, but the difference between them is not significant. CD3^＋ ,CD4^＋ were lower and IL-4, TNF-α were higher in COPD group than those in control group, which means that abnormal cellular immunity function and inflammatory process still existed in the stable period of COPD.