单克隆抗体检测囊虫病人循环抗原及其在疗效考核中的应用

DETECTION OF CIRCULATING ANTIGEN IN PATIENTS WITH CYSTICERCOSIS BY RIHA AND ITS APPLICATION IN THE EVALUATION OF CURATIVE EFFECTS

用单克隆抗体致敏绵羊红细胞作反向间接血凝试验，检测７７例囊虫病人血清循环抗原，阳性率为６４．９％；３０例正常人血清的假阳性率为０；２２例华支睾吸虫病人、２４例包虫病人和１７例弓形虫病人的交叉反应率分别为４．６％、４．２％和１１．８％。结果表明，该法较为敏感、特异，且稳定可靠、简单易行，尤适于在基层医疗单位推广应用。检测经治疗１～１０个疗程囊虫病人血清中循环抗原和抗体，发现随着疗程的增加循环抗原的消失明显快于抗体，且大部分患者的循环抗原和其临床症状、体征同时消失。表明循环抗原的检测对囊虫病的诊断和疗效考核有一定的实用价值。

This paper reported the detection of circulating antigen(CAg) in sera of patients with cysticercosis by RIHA, in which the sheeps' RBCs were sensitized with McAb. The positive rate in 77 patients with cysticercosis was 64.9%. In 30 healthy persons, no false positive was found. In 24 patients with echinococcosis (22 with clonorchiasis and 17 with toxoplasmosis), the positive rates were 4.2%, 4.6%and 11.8%respectively. Results showed that this method was sensitive, specific and easy to operate. It may be used in foundamental medical units. The CAg and antibody(Ab) were detected in the sera from the patients with cysticercosis after 1 to 10 treatment courses. With increase of the courses, the negative rates of the CAg were clearly higher than those of Ab, and with disappearance of the CAg, most patients' symptoms and signs disappeared, too. Results also showed that detection of CAg had some applicable values in the diagnosis and evaluation of curative effects of patients with cysticercosis.